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Regeneration of human auditory nerve. In vitro/in video demonstration of neural progenitor cells in adult human and guinea pig spiral ganglion

Rask-Andersen, Helge ; Boström, Marja ; Gerdin, Bengt ; Kinnefors, Anders ; Nyberg, Gunnar ; Engstrand, Thomas ; Miller, Josef M ; Lindholm, Dan

Hearing Research, 2005, Vol.203(1), pp.180-191 [Peer Reviewed Journal]

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  • Title:
    Regeneration of human auditory nerve. In vitro/in video demonstration of neural progenitor cells in adult human and guinea pig spiral ganglion
  • Author/Creator: Rask-Andersen, Helge ; Boström, Marja ; Gerdin, Bengt ; Kinnefors, Anders ; Nyberg, Gunnar ; Engstrand, Thomas ; Miller, Josef M ; Lindholm, Dan
  • Language: English
  • Subjects: Spiral Ganglion ; In Vitro Culture ; Regeneration ; Stem Cells ; Human ; Spiral Ganglion ; In Vitro Culture ; Regeneration ; Stem Cells ; Human ; Medicine ; Anatomy & Physiology
  • Is Part Of: Hearing Research, 2005, Vol.203(1), pp.180-191
  • Description: Time lapse video recordings of cultured adult human and guinea pig spiral ganglion (hSG and gpSG) show that mitogen responsive progenitor/stem cells develop in the form of spheres that proliferate and differentiate into mature neurons and glia cells. Neurospheres, cultured with EGF and bFGF showed expression of nestin and incorporation of 5′-Bromo-2-deoxyuridine (BrdU). Newly formed BrdU labelled cells were positive for β-tubulin, and also for GFAP demonstrating that neuronal cells were derived from a dividing population of progenitor cells. Dissociated spheres cultured either with glia cell line-derived neurotrophic factor (GDNF) or brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3), induced differentiation of the progenitor cells. Video microscopy showed that neurons develop from subcultured spheres maintained for up to four weeks. Neurons showed fasciculation and migration with a speed of 10–30 μm/h, and some cells had up to 6 mm long neurites coexpressing TrkB...
  • Identifier: ISSN: 0378-5955 ; E-ISSN: 1878-5891 ; DOI: 10.1016/j.heares.2004.12.005