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Keratinocytes Inhibit Expression of Connective Tissue Growth Factor in Fibroblasts In Vitro by an Interleukin-1α-Dependent Mechanism

Nowinski, Daniel ; Höijer, Patrik ; Engstrand, Thomas ; Gerdin, Bengt ; Ivarsson, Mikael ; Rubin, Kristofer

Journal of Investigative Dermatology, August 2002, Vol.119(2), pp.449-455 [Peer Reviewed Journal]

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  • Title:
    Keratinocytes Inhibit Expression of Connective Tissue Growth Factor in Fibroblasts In Vitro by an Interleukin-1α-Dependent Mechanism
  • Author/Creator: Nowinski, Daniel ; Höijer, Patrik ; Engstrand, Thomas ; Gerdin, Bengt ; Ivarsson, Mikael ; Rubin, Kristofer
  • Language: English
  • Subjects: Coculture ; Re-Epithelialization ; Transforming Growth Factor Β1 ; Wound Healing ; Medicine
  • Is Part Of: Journal of Investigative Dermatology, August 2002, Vol.119(2), pp.449-455
  • Description: The wound healing process concludes with downregulation of fibroblast activity. Clinical observations suggest that the regenerating epidermis suppresses this activity. An important regulator of fibroblast activity is the fibrogenic cytokine connective tissue growth factor. We hypothesized that epidermal keratinocytes may affect fibroblast activity via this cytokine. We demonstrate keratinocyte-mediated suppression of connective tissue growth factor at both the mRNA and protein levels by around 50% or more when fibroblasts were cultured in multiwell plates with keratinocyte cultures in accompanying semipermeable cell culture inserts, or stimulated by keratinocyte-conditioned media. Both basal and transforming-growth-factor-β1-stimulated levels of connective tissue growth factor were inhibited. A 3 h coculture period with keratinocytes was sufficient to suppress connective tissue growth factor expression by fibroblasts, but the inhibition developed over a time period of around 16...
  • Identifier: ISSN: 0022-202X ; E-ISSN: 1523-1747 ; DOI: 10.1046/j.1523-1747.2002.01841.x