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Chlamydophila psittaci DNA Detection in the Faeces of Cage Birds

Sareyyupoglu, B. ; Cantekin, Z. ; Bas, B.

Zoonoses and Public Health, September 2007, Vol.54(6‐7), pp.237-242 [Peer Reviewed Journal]

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  • Title:
    Chlamydophila psittaci DNA Detection in the Faeces of Cage Birds
  • Author/Creator: Sareyyupoglu, B. ; Cantekin, Z. ; Bas, B.
  • Subjects: Cage Birds ; Chlamydophila Psittaci ; Faeces ; Polymerase Chain Reaction
  • Is Part Of: Zoonoses and Public Health, September 2007, Vol.54(6‐7), pp.237-242
  • Description: In this study, we investigated the shedding of in faecal samples from cage birds using PCR testing. A total of 47 faeces samples were collected from four different aviaries. Main symptoms determined after clinical investigation and owner histories of the birds showed that the birds had respiratory system problems changing from mild to severe. They also showed conjunctivitis, diarrhoea or no symptoms at all. DNA extractions from faeces were performed with the QIAamp DNA Stool Mini Kit. Following PCR with specific primers, 43 (91.5%) samples were determined to harbour‐specific DNA. Only one bird from each aviary was found to be negative by PCR. As all the samples from birds showing clinical signs were PCR positive, these signs could be correlated to psittacosis in these birds. shedding in faeces was detected in all the aviaries. After restriction analysis of PCR amplicons with I enzyme, all the isolates showed the same RFLP (Restriction Fragment Length Polymorphism) patterns with the control DNA. PCR following QIAamp DNA stool mini kit extraction of faecal samples was found to be a rapid, specific, sensitive, reproducible test, which did not need additional nested PCR of samples.
  • Identifier: ISSN: 1863-1959 ; E-ISSN: 1863-2378 ; DOI: 10.1111/j.1863-2378.2007.01060.x