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Impact of enzymatic hydrolysis on the quantification of total urinary concentrations of chemical biomarkers

Dwivedi, Prabha ; Zhou, Xiaoliu ; Powell, Tolar G ; Calafat, Antonia M ; Ye, Xiaoyun

Chemosphere, May 2018, Vol.199, pp.256-262 [Peer Reviewed Journal]

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  • Title:
    Impact of enzymatic hydrolysis on the quantification of total urinary concentrations of chemical biomarkers
  • Author/Creator: Dwivedi, Prabha ; Zhou, Xiaoliu ; Powell, Tolar G ; Calafat, Antonia M ; Ye, Xiaoyun
  • Language: English
  • Subjects: Human Biomonitoring ; Solid Phase Extraction-LC-MS ; Β-Glucuronidase Enzyme Hydrolysis ; Environmental Exposure ; Triclocarban ; Parabens ; Personal Care Products ; Chemistry ; Ecology
  • Is Part Of: Chemosphere, May 2018, Vol.199, pp.256-262
  • Description: Human exposure to consumer and personal care products chemicals such as phenols, including parabens and other antimicrobial agents, can be assessed through biomonitoring by quantifying urinary concentrations of the parent chemical or its metabolites, often after hydrolysis of phase II conjugates. Developing suitable analytical methods for the concurrent quantification of multiple exposure biomarkers is challenging because optimal conditions for the hydrolysis of such conjugates (e.g., O-glucuronides, -glucuronides, sulfates) may differ depending on the biomarker. We evaluated the effectiveness of seven commercial hydrolytic enzymes to simultaneously hydrolyze -glucuronides (using the antibacterial triclocarban as example compound) and other conjugates (using select phenols and parabens as examples) by using on-line solid phase extraction–high performance liquid chromatography-isotope dilution-tandem mass spectrometry. Incubation (30 min,...
  • Identifier: ISSN: 0045-6535 ; E-ISSN: 1879-1298 ; DOI: 10.1016/j.chemosphere.2018.01.177